A scientific diagram illustrating a biological protocol, showing steps like isolating bone marrow from mice femurs, BMDM infection, incubation with M-CSF, adding gentamicin, PBS washing, and counting CFUs. The style is a clear, cartoon-like illustration.

Scientific diagram explaining this protocol: Two mice per batch of BMDMs are sacrificed, then bone marrow is harvested from their femurs and tibias Cells are differentiated for 7 days via incubation with M-CSF BMDMs are infected at an MOI of 10 1 hour after inoculation, gentamicin is added to culture media to kill any non-phagocytosed bacteria 30 minutes later, cells are washed with PBS to remove gent then dissociated and collected Finally, surviving BMDMs are counted and CFUs enumerated See more